Which molecule serves as the template for mRNA synthesis during transcription in a typical eukaryotic cell?

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Prepare for the A2 Genetic Control of Proteins and Control of Gene Expression Exam. Study with in-depth flashcards and multiple-choice questions that offer hints and explanations. Ace your exam!

Multiple Choice

Which molecule serves as the template for mRNA synthesis during transcription in a typical eukaryotic cell?

Explanation:
During transcription, RNA polymerase uses a DNA strand as the guide to build the RNA. The template is the DNA template strand, which is read in the 3' to 5' direction, while the mRNA is synthesized in the 5' to 3' direction. This results in an RNA sequence that is complementary to the template strand and nearly identical to the coding (non-template) DNA strand, except that uracil replaces thymine. The coding strand is not used as the template. Therefore, the DNA template strand is the molecule that serves as the template for mRNA synthesis.

During transcription, RNA polymerase uses a DNA strand as the guide to build the RNA. The template is the DNA template strand, which is read in the 3' to 5' direction, while the mRNA is synthesized in the 5' to 3' direction. This results in an RNA sequence that is complementary to the template strand and nearly identical to the coding (non-template) DNA strand, except that uracil replaces thymine. The coding strand is not used as the template. Therefore, the DNA template strand is the molecule that serves as the template for mRNA synthesis.

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